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PubMed · 4019521

Why does Coomassie Brilliant Blue R interact differently with different proteins? A partial answer.

M Tal·A Silberstein·E Nusser
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Abstract

Dimethyl sulfoxide was found to be effective for extraction of Coomassie Brilliant Blue R-250 (Coomassie R) from stained proteins on polyacrylamide gel slices. A good correlation was found between the ability of different proteins to bind Coomassie R and their capacity for interaction with Coomassie Brilliant Blue G-250 (Coomassie G) in solution. Scatchard analysis showed that the number of Coomassie R ligands bound to each protein molecule is approximately proportional to the number of positive charges on the protein, about 1.5-3 dye molecules/charge.

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Electrophoresis, Polyacrylamide GelKineticsPeptidesProteinsRosaniline DyesStructure-Activity RelationshipComparative StudyJournal Article

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M Tal, A Silberstein, E Nusser. 1985-08-25. Why does Coomassie Brilliant Blue R interact differently with different proteins? A partial answer.. https://pubmed.ncbi.nlm.nih.gov/4019521/

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