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PubMed · 4028112

Graphical method for analysing stathmokinetic data.

J L Roti Roti
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Abstract

The accumulation of cells in G2M measured flow cytometrically follows a straight line when plotted against time in colcemid with the transform ln (1 + fG2M), where fG2M is the fraction of cells in the G2M peak. Extending this function to the value, ln 2, will locate a point B. Drawing a line from point B to ln 1 perpendicular to the abscissa, will locate point A at the initial value of ln (1 + fG2M). A line from ln 2 at t = 0 through point A will intersect the abscissa at the cell doubling time. Application of this method to the depletion of G1 cells is also possible.

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AnimalsCell CycleCells, CulturedCricetinaeDemecolcineFlow CytometryHumansInterphaseMitosisJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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J L Roti Roti. 1985. Graphical method for analysing stathmokinetic data.. https://doi.org/10.1111/j.1365-2184.1985.tb00697.x

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