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PubMed · 4035359

Z-DNA forms without an alternating purine-pyrimidine sequence in solution.

J Feigon·A H Wang·G A van der Marel·J H van Boom·A Rich
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Abstract

Nuclear magnetic resonance spectra (proton and phosphorus-31) and ultraviolet absorption spectra of the DNA decamer d(br5CGbr5CGATbr5CGbr5CG), in which the central two adenine-thymine base pairs are out of order with the rest of the purine-pyrimidine alternation sequence, indicate that under appropriate solvent conditions (high salt and methanol) the molecule undergoes a structural transition from a right-handed B-DNA conformation to a left-handed Z-DNA conformation. Measurements of the two-dimensional nuclear Overhauser effect on the decamer indicate that all of the guanines as well as the two equivalent thymines adopt the syn conformation.

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Base SequenceDNAMagnetic Resonance SpectroscopyNucleic Acid ConformationSpectrophotometry, UltravioletJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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J Feigon, A H Wang, G A van der Marel, J H van Boom, A Rich. 1985-10-04. Z-DNA forms without an alternating purine-pyrimidine sequence in solution.. https://doi.org/10.1126/science.4035359

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