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PubMed · 4060593

Switch-on of transcriptase function in reovirus: analysis of polypeptide changes using 2-D gels.

D D Ewing·M D Sargent·J Borsa
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Abstract

Two-dimensional gel electrophoresis (IEF and SDS-PAGE) was used to examine virion polypeptide changes associated with switch-on of transcriptase function in reovirus. Results reveal that switch-on is correlated with altered electrophoretic behavior of a specific minor polypeptide (delta 1) which is present in intermediate subviral particles. A second finding is that each of the molecular weight classes of viral polypeptides exists as a series of subspecies with different isoelectric points. This suggests that extensive posttranslational modification of progeny viral polypeptides occurs during particle morphogenesis. These findings have important theoretical and practical implications.

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DNA-Directed RNA PolymerasesElectrophoresis, Polyacrylamide GelEnzyme ActivationGene Expression RegulationIsoelectric PointKineticsMammalian orthoreovirus 3Molecular WeightMorphogenesisProtein Processing, Post-TranslationalReoviridaeViral ProteinsJournal Article

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D D Ewing, M D Sargent, J Borsa. 1985-07-30. Switch-on of transcriptase function in reovirus: analysis of polypeptide changes using 2-D gels.. https://doi.org/10.1016/0042-6822(85)90285-5

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