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Nucleosome core particle structure and structural changes in solution.

K O Greulich·J Ausio·H Eisenberg
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Abstract

The radius of gyration, Rg, of chicken erythrocyte nucleosome core particles, was found to be 4.56 (+/- 0.07) nm by small-angle X-ray scattering, independent of particle concentration and of NaCl concentration between 0.1 M and 0.6 M-NaCl. The large, positive, second virial coefficient, A2, from particle concentration dependence (but independent of NaCl concentration) in small-angle X-ray scattering may indicate non-electrostatic repulsive ordering over large distances. Density contrast variation in equilibrium sedimentation with a small probe (sucrose) and a larger probe (gamma-cyclodextrin) yields good results for core particle hydration in the first instance, and for an estimate of the total particle volume in the second instance.

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AnimalsChickensErythrocytesMathematicsMolecular WeightNucleosomesSolutionsUltracentrifugationX-Ray DiffractionJournal ArticleResearch Support, Non-U.S. Gov't

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K O Greulich, J Ausio, H Eisenberg. 1985-11-05. Nucleosome core particle structure and structural changes in solution.. https://doi.org/10.1016/0022-2836(85)90266-9

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