Solid-phase synthesis of oligoribonucleotides.

I Hirao; M Ishikawa; K Miura

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PubMed · 4088866

Solid-phase synthesis of oligoribonucleotides.

I Hirao·M Ishikawa·K Miura

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Abstract

Selective deprotection of the 5'-O-dimethoxytrityl group of oligoribonucleotides required for 5'-deprotection reaction during synthesis of an oligoribonucleotide was achieved by the treatment with 1% dichloroacetic acid in dichloromethane at room temperature, without removal of the 2'-O-tetrahydropyranyl group. Phosphorylation of protected ribonucleosides and coupling reaction to the 5' end of oligoribonucleotides attached to polystyrene solid support were carried out by the use of bifunctional reagent 2-chlorophenyl-O-O-bis(1-benzotriazolyl) phosphate. In this way, trinucleotides; TpTpT, dUpdUpT, and UpUpT, were synthesized.

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Chromatography, High Pressure Liquid Drug Stability Indicators and Reagents Oligoribonucleotides Structure-Activity Relationship Journal Article

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I Hirao, M Ishikawa, K Miura. 1985. Solid-phase synthesis of oligoribonucleotides.. https://pubmed.ncbi.nlm.nih.gov/4088866/

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