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PubMed · 4089815

Soluble crosslinked fibrin(ogen) polymers.

E Selmayr·G Müller-Berghaus
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Abstract

The present study is concerned with the formation of fibrin-fibrinogen associations in the presence of FXIIIa while fibrinolysis was inhibited by aprotinin and EACA. SDS agarose-polyacrylamide gel electrophoresis on 2.5% gels under non-reducing conditions and ultracentrifugation of the associations on urea-sucrose density gradients showed the formation of soluble cross-linked high molecular weight (HMW) fibrin-fibrinogen polymers with an estimated molecular size up to 10 times that of fibrinogen. After incubation of a mixture of 131I-fibrinogen (4 mg/ml) and 125I-desAA-fibrin (0.2 mg/ml) with FXIIIa (2 U/ml) for 1 h at 37 degrees C, about 5% of the fibrinogen and 80% of the fibrin was incorporated into the generated soluble HMW polymers. The detection of soluble crosslinked fibrin-fibrinogen polymers could be a useful diagnostic criterion for imminent DIC.

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Disseminated Intravascular CoagulationFibrinFibrinogenHumansIn Vitro TechniquesMolecular WeightPolymersProtein ConformationSolubilityJournal ArticleResearch Support, Non-U.S. Gov't

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E Selmayr, G Müller-Berghaus. 1985-12-17. Soluble crosslinked fibrin(ogen) polymers.. https://pubmed.ncbi.nlm.nih.gov/4089815/

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