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PubMed · 4091274

Competitive binding assay for drugs that interfere with calmodulin function.

H Le Vine·N Sahyoun·P Cuatrecasas
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Abstract

A direct 125I-calmodulin-binding system utilizing a particulate cytoskeletal preparation of the neuronal calmodulin-dependent protein kinase type II provides a simple, rapid filtration-based method for assessing anti-calmodulin drug activity. The binding assay avoids potential artifacts due to direct drug effects on the standard phosphodiesterase enzyme assay while obtaining comparable IC50 values for a spectrum of drugs. It also provides a tool to probe mechanisms of action of putative anticalmodulin agents which may help elucidate selective pharmacologic modifications of pathophysiological processes.

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AnimalsBinding, CompetitiveBrainCalmodulinCytoskeletonIn Vitro TechniquesKineticsNeuronsProtein KinasesRatsTrifluoperazineJournal Article

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H Le Vine, N Sahyoun, P Cuatrecasas. 1985-11-15. Competitive binding assay for drugs that interfere with calmodulin function.. https://doi.org/10.1016/0003-2697(85)90065-x

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