PubMed · 4097553
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W Liu. 1970. A simplified cytologic staining technic.. https://doi.org/10.1093/ajcp%2F54.5.767
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This paper reviews groundwater pollution caused by the disposal of untreated effluents of a dye factory located 20 km to the west of Nicosia (Cyprus). The task of the work was to investigate the nature of the pollution and differentiate it from other possible pollution sources in the area. It focused on toxicity testing and biofractionation in order to address the most toxic pollutants and, on the evaluation of GC/FID profiles for investigating the connection between groundwater pollution and the effluents. This connection was successful due to a multiple comparison amongst the GC/FID profiles resulting from the polluted groundwater, the water from the reference areas and the dye effluents.
A method is described that allows quantitative determination of polyethylene glycol (PEG) concentrations by spectrophotometric measurement of fluorescein dye absorbance after its partitioning into an aqueous two-phase system containing mPEG (M(r) 5 kDa) in the upper phase and ammonium sulfate in the lower phase. The absorbance decrease of fluorescein in the lower phase is directly proportional to the mPEG concentration, with two proportionality constants equal to 4.42 x 10(5) and 2.84 x 10(5) M-1 cm-1 in the range of 0-0.4 and 0.4-1 microM, respectively. This experimental technique can be extended to PEGs of other molecular weights by means of calibration curves that give for each size of PEG the adequate proportionality constants. The results indicate that the quantitative determination is not affected by the presence of many substances such as proteins, reducing agents, and salts, at the usual concentrations.
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