PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 40993210

A consensus guide to preclinical indirect calorimetry experiments.

Alexander S Banks·David B Allison·Thierry Alquier·Ansarullah·Steven N Austad·Johan Auwerx·Julio E Ayala·Joseph A Baur·Stefania Carobbio·Gary A Churchill·Morten Dall·Rafael de Cabo·Jose Donato·Nathalia R V Dragano·Carol F Elias·Anthony W Ferrante·Brian N Finck·Jose E Galgani·Zachary Gerhart-Hines·Laurie J Goodyear·Justin L Grobe·Rana K Gupta·Kirk M Habegger·Sean M Hartig·Andrea L Hevener·Steven B Heymsfield·Corey D Holman·Martin Hrabě de Angelis·David E James·Lawrence Kazak·Jae Bum Kim·Martin Klingenspor·Xingxing Kong·Sander Kooijman·Louise Lantier·K C Kent Lloyd·James C Lo·Irfan J Lodhi·Paul S MacLean·Owen P McGuinness·Gema Medina-Gómez·Raghavendra G Mirmira·Christopher D Morrison·Gregory J Morton·Timo D Müller·Yoshihiro Ogawa·David Pajuelo-Reguera·Matthew J Potthoff·Nathan Qi·Marc L Reitman·Patrick C N Rensen·Jan Rozman·Jennifer M Rutkowsky·Kei Sakamoto·Philipp E Scherer·Gary J Schwartz·Radislav Sedlacek·Mohammed Selloum·Saame Raza Shaikh·Shuai Chen·Gerald I Shulman·Vojtěch Škop·Alexander A Soukas·John R Speakman·Bruce M Spiegelman·Gregory R Steinberg·Katrin J Svensson·John P Thyfault·Tony Tiganis·Paul M Titchenell·Nigel Turner·Licio A Velloso·Antonio Vidal-Puig·Christopher S Ward·Ashley S Williams·Christian Wolfrum·Allison W Xu·Ying Xu·Juleen R Zierath·International Indirect Calorimetry Consensus Committee (IICCC)
View original source ↗Export metadata ↓

Abstract

Understanding the complex factors influencing mammalian metabolism and body weight homeostasis is a long-standing challenge requiring knowledge of energy intake, absorption and expenditure. Using measurements of respiratory gas exchange, indirect calorimetry can provide non-invasive estimates of whole-body energy expenditure. However, inconsistent measurement units and flawed data normalization methods have slowed progress in this field. This guide aims to establish consensus standards to unify indirect calorimetry experiments and their analysis for more consistent, meaningful and reproducible results. By establishing community-driven standards, we hope to facilitate data comparison across research datasets. This advance will allow the creation of an in-depth, machine-readable data repository built on shared standards. This overdue initiative stands to markedly improve the accuracy and depth of efforts to interrogate mammalian metabolism. Data sharing according to established best practices will also accelerate the translation of basic findings into clinical applications for metabolic diseases afflicting global populations.

Explore related subjects

Calorimetry, IndirectHumansEnergy MetabolismAnimalsJournal ArticleReviewConsensus Statement

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Alexander S Banks, David B Allison, Thierry Alquier, Ansarullah, Steven N Austad, Johan Auwerx, Julio E Ayala, Joseph A Baur, Stefania Carobbio, Gary A Churchill, Morten Dall, Rafael de Cabo, Jose Donato, Nathalia R V Dragano, Carol F Elias, Anthony W Ferrante, Brian N Finck, Jose E Galgani, Zachary Gerhart-Hines, Laurie J Goodyear, Justin L Grobe, Rana K Gupta, Kirk M Habegger, Sean M Hartig, Andrea L Hevener, Steven B Heymsfield, Corey D Holman, Martin Hrabě de Angelis, David E James, Lawrence Kazak, Jae Bum Kim, Martin Klingenspor, Xingxing Kong, Sander Kooijman, Louise Lantier, K C Kent Lloyd, James C Lo, Irfan J Lodhi, Paul S MacLean, Owen P McGuinness, Gema Medina-Gómez, Raghavendra G Mirmira, Christopher D Morrison, Gregory J Morton, Timo D Müller, Yoshihiro Ogawa, David Pajuelo-Reguera, Matthew J Potthoff, Nathan Qi, Marc L Reitman, Patrick C N Rensen, Jan Rozman, Jennifer M Rutkowsky, Kei Sakamoto, Philipp E Scherer, Gary J Schwartz, Radislav Sedlacek, Mohammed Selloum, Saame Raza Shaikh, Shuai Chen, Gerald I Shulman, Vojtěch Škop, Alexander A Soukas, John R Speakman, Bruce M Spiegelman, Gregory R Steinberg, Katrin J Svensson, John P Thyfault, Tony Tiganis, Paul M Titchenell, Nigel Turner, Licio A Velloso, Antonio Vidal-Puig, Christopher S Ward, Ashley S Williams, Christian Wolfrum, Allison W Xu, Ying Xu, Juleen R Zierath, International Indirect Calorimetry Consensus Committee (IICCC). 2025-09-24. A consensus guide to preclinical indirect calorimetry experiments.. https://doi.org/10.1038/s42255-025-01360-4

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2006

Indirect calorimetry: relevance to patient outcome.

Indirect calorimetry provides an important adjunctive monitor for the provision of nutrition support in the critically ill patient. Accuracy in determining caloric requirements may serve to optimize benefit from nutrition therapy and improve patient outcome. A number of strategies in nutrition management in the intensive care setting (eg, dosing of enteral nutrition, monitoring cumulative caloric balance, and deliberate but "permissive" underfeeding) necessitate the determination of a fairly specific goal for caloric provision. Inaccuracy leading to inappropriate under- or overfeeding may generate additional morbidity and adverse clinical consequences for patients already at high risk from hypermetabolic stress response to injury.

Calorimetry, Indirect↗
PubMed2006

Indirect calorimetry: methodology, instruments and clinical application.

PURPOSE OF REVIEW: This review aims to identify the basic methods for accurately measuring a patient's energy expenditure in clinical nutrition practice by indirect calorimetry, and the impact upon a disease state of applying the results obtained. RECENT FINDINGS: The open-circuit method is the most widely used in the majority of classical instruments for measuring energy consumption. Advances in gas exchange measurement have made this technique readily and precisely available at the bedside. Nevertheless, it is important to understand its intricate primary methodology for safe and correct application. The stress and activity factors should be carefully and specifically applied, and the respiratory quotient abandoned, for tailoring a patient's daily nutrition regimens. Caloric expenditure measured by indirect calorimetry coupled with the doubly labeled water technique introduced the concept of physical activity energy expenditure, which added to resting energy expenditure results in total daily energy expenditure. Compact modular and handheld devices have been introduced into the market, together with similar technology for evaluating exercise energy expenditure, making utilization easier, safer and precise. In the critically ill population, which is exposed to medical and surgical interventions, indirect calorimetry has greatly changed the practice of caloric administration, significantly reducing the total daily amount. SUMMARY: In conclusion, one has to be careful when choosing devices, and understanding and clinically applying the results obtained by indirect calorimetry, bearing in mind that measured resting energy expenditure should be the daily caloric goal in order to diminish clinical morbidity.

Calorimetry, Indirect↗
PubMed2006

[Resting energy expenditure during hemodialysis].

Very few studies have so far reported about resting energy expenditure (REE) in chronic renal failure and there is no information available on REE during hemodialysis (HD). Hypothetically, we can expect an increase in REE during HD procedure (due to the inflammatory response to extracorporeal blood circuit). However, such increase in REE could be modified by thermal balance of the procedure. In our study, REE was measured by indirect calorimetry (Deltatrac Datex) in a group of 13 HD patients (7 males and 6 females, mean age 59.8 +/- 13.5 years). In each patient, REE was assessed during two HD sessions: one isothermic and one thermoneutral. All other HD parameters were kept constant. The control group consisted of 14 healthy subjects (4 males and 10 females, mean age 41.3 +/- 20.5 years) with normal renal function. There was a significant difference in thermal balance between the two HD settings: -199 kJ/HD in isothermic and -4kJ/HD in thermoneutral HD sessions (p < 0.01). Measured REE values obtained in HD patients before HD session (7 316 +/- 919 kJ/day/1.73 m2) did not differ significantly from those of the healthy controls (7 264 +/- 1 016 kJ/day/1.73 m2). Similarly, there was no significant difference in calculated EE values (Harris-Benedict equation). In the 10th minute of the HD session, there was a slight, transitory decrease in REE (mean decrease by 3.2% during isothermic and by 2.8% during thermoneutral HD session, ns). In the 70th minute, REE returned to pre-dialysis values. After a light meal in the 110th minute REE increased by 8% during isothermic and by 6.3% during thermoneutral HD session. At the end of the HD session (i.e. in the 215th minute) REE again returned to pre-dialysis values. Intra-dialysis changes in REE were similar in both isothermic and thermoneutral HD sessions. The results of our study did not confirm the expected influence of HD procedure on REE in the two different thermal HD settings. We conclude that there is no significant difference between REE in HD patients and healthy controls and that REE values are not significantly influenced by hemodialysis procedure.

Calorimetry, Indirect↗