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Enzyme assay in microsomes below zero degrees.

P Debey·C Balny·P Douzou
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Abstract

Reactions of a membrane-bound multienzyme complex (electron-transport chain of rat-liver microsomes) suspended in aqueous-organic solvent used as antifreeze at temperatures below 0 degrees were studied. In the presence of a relatively high concentration of ethylene glycol, electron transfer can still be observed and some individual and sequential enzyme assays can be performed over a wide range of temperatures below 0 degrees .

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AnimalsCytochrome P-450 Enzyme SystemCytochrome ReductasesCytochrome c GroupElectron TransportEthylenesFreezingGlycolsIn Vitro TechniquesKineticsMaleMicrosomes, LiverMultienzyme ComplexesNADPOxidoreductasesRatsSpectrophotometryJournal Article

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P Debey, C Balny, P Douzou. 1973. Enzyme assay in microsomes below zero degrees.. https://doi.org/10.1073/pnas.70.9.2633

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