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Control of protein and energy intake: role of plasma amino acids and brain neurotransmitters.

G H Anderson
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Amino AcidsAnimalsAppetite RegulationBrainCatecholaminesDietary ProteinsEnergy MetabolismFeeding BehaviorHumansNeurotransmitter AgentsSerotoninTime FactorsJournal ArticleReview

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G H Anderson. 1979. Control of protein and energy intake: role of plasma amino acids and brain neurotransmitters.. https://doi.org/10.1139/y79-158

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Chimeric structural isomer fragments as cost-efficient internal standards for amino acid quantification by mass spectrometry.

Amino acid (AA) profiles from body fluids such as blood and urine are clinical indicators for diagnosing metabolic and hepatic diseases. Current quantitative methods, such as liquid chromatography-mass spectrometry (LC-MS) with isotopically labelled internal standards (ISs), are costly and technically demanding. This study proposes a cost-efficient alternative using structural isomers as ISs in a direct liquid infusion (DLI) tandem mass spectrometry (MS/MS) approach. The method leverages chimeric spectra and fragment intensity ratios to quantify AAs, demonstrating high linearity and precision even with a 3D ion trap mass analyser. This approach offers a viable strategy for AA quantification in preventive medicine, particularly for screening metabolic diseases such as phenylketonuria, diabetes, and liver dysfunction.

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