An efficient method to produce specific anti-actin.

B M Jockusch; K H Kelley; R K Meyer; M M Burger

doi:10.1007/bf00495757

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An efficient method to produce specific anti-actin.

B M Jockusch·K H Kelley·R K Meyer·M M Burger

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Abstract

The production of affinity column purified (specific) anti-actin is described. With the immunization scheme employed, all rabbits produced precipitating antibodies over several months, so that 30 mg specific anti-actin per rabbit could be isolated in 6 months. The antibodies against native and detergent denatured smooth muscle actin are characterized by immunodiffusion tests, staining of the I-band of isolated myofibrils and stress fibers in tissue culture cells, using indirect immunofluorescence.

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Actins Animals Antibody Formation Chickens Chromatography, Affinity Fluorescent Antibody Technique Immune Sera Immunodiffusion Rabbits Journal Article

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B M Jockusch, K H Kelley, R K Meyer, M M Burger. 1978-04-04. An efficient method to produce specific anti-actin.. https://doi.org/10.1007/bf00495757

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