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PubMed · 4177332

Developments in immunofluorescence: the need for standardization.

G D Johnson·E H Beutner·E J Holborow
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Abstract

DIFFICULTIES EXPERIENCED BY THE NEWCOMER TO THE FLUORESCENT ANTIBODY STAINING METHOD LARGELY ARISE FROM TWO CAUSES: (1) the many modifications in the procedure which have been suggested over the last decade; (2) the variability of the basic reagent, fluorescent conjugate, in terms of potency and specificity. The ensuing problem of non-reproducibility is clearly an important factor for clinical laboratories now faced with increasing demands for routine tests involving immunofluorescence. In this paper a simple approach to standardization is described which, if adopted, would lead to a more efficient use of the method.

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Antigen-Antibody ReactionsDrug StorageFluoresceinsFluorescent Antibody TechniqueFreeze DryingHumansImmune SeraMicroscopy, FluorescenceStaining and LabelingJournal Article

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G D Johnson, E H Beutner, E J Holborow. 1967. Developments in immunofluorescence: the need for standardization.. https://doi.org/10.1136/jcp.20.5.720

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