Allelic variation alters expression and antigen presentation of MR1 allomorphs.

Adam G Nelson; Victoria Letoga; Clarice Z Q Lee; Carolyn Samer; Songyi Li; Lucy J Meehan; Hamish E G McWilliam; Nicholas A Gherardin; Allison Abendroth; Jose A Villadangos; Barry Slobedman; Alexandra J Corbett; James McCluskey; Jamie Rossjohn; Zhenjun Chen; Michael N T Souter; Wael Awad

doi:10.1016/j.jbc.2026.111470

PubMed · 42001947

Allelic variation alters expression and antigen presentation of MR1 allomorphs.

Adam G Nelson·Victoria Letoga·Clarice Z Q Lee·Carolyn Samer·Songyi Li·Lucy J Meehan·Hamish E G McWilliam·Nicholas A Gherardin·Allison Abendroth·Jose A Villadangos·Barry Slobedman·Alexandra J Corbett·James McCluskey·Jamie Rossjohn·Zhenjun Chen·Michael N T Souter·Wael Awad

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Abstract

The major histocompatibility complex (MHC) class I-related protein 1 (MR1) presents vitamin B-derived metabolites to mucosal-associated invariant T (MAIT) and other T cells. There is limited polymorphism of MR1, the functional impact of which is not understood. We examined the impact of allelic variation of MR1 on the expression, structure and function of the known MR1 allomorphs. The expression and function of MR1∗02, MR1∗03, and MR1∗06 were similar to the canonical MR1∗01. Crystal structures of four MR1 allomorphs show that their polymorphisms do not impact the three-dimensional fold of MR1. Despite the binding of 5-OP-RU to MR1∗05 and its cell surface upregulation, this allomorph was severely impaired in its ability to activate primary MAIT cells. This phenotype was controlled by two (His90Gln and Glu52Gly) of its three polymorphisms, which led to the loss of structurally stabilizing interactions. When cells expressing the MR1 allomorphs were infected with herpes simplex virus type 1 (HSV-1), the nascent expression of all allomorphs was severely impaired, but surface expression of MR1∗04:01 and MR1∗04:02 was relatively less impacted. Hence, MR1 allelic variation alters the expression and function of the MR1∗04 and MR1∗05 allomorphs, with implications for MAIT cell and diverse MR1-reactive T cell immunity.

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Humans Alleles Histocompatibility Antigens Class I Minor Histocompatibility Antigens Antigen Presentation Polymorphism, Genetic Mucosal-Associated Invariant T Cells Animals MAIT MR1 allele antigen polymorphism Journal Article Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural Research Support, U.S. Gov't, P.H.S.

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Adam G Nelson, Victoria Letoga, Clarice Z Q Lee, Carolyn Samer, Songyi Li, Lucy J Meehan, Hamish E G McWilliam, Nicholas A Gherardin, Allison Abendroth, Jose A Villadangos, Barry Slobedman, Alexandra J Corbett, James McCluskey, Jamie Rossjohn, Zhenjun Chen, Michael N T Souter, Wael Awad. 2026-04-17. Allelic variation alters expression and antigen presentation of MR1 allomorphs.. https://doi.org/10.1016/j.jbc.2026.111470

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