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PubMed · 4201682

Phosphate-induced protein chromatography.

R A Rimerman·G W Hatfield
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Abstract

High phosphate concentration is shown to cause a large proportion of the proteins of a cell-free extract of Escherichia coli to bind to agarose columns to which L-valine is attached. With a decreasing concentration gradient of potassium phosphate, the proteins elute in relation to their solubility in concentrated ammonium sulfate. This column technique appears to provide a general tool for the purification of proteins.

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Bacillus subtilisCell-Free SystemChromatography, GelEscherichia coliGalactosidasesHydro-LyasesMethodsPhosphatesPolysaccharidesPotassiumProteinsSolubilityStreptomycinThreonineValineJournal Article

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R A Rimerman, G W Hatfield. 1973-12-21. Phosphate-induced protein chromatography.. https://doi.org/10.1126/science.182.4118.1268

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