Chromosome-level de novo assembly of the nuclear and mitochondrial genomes of Arcopilus aureus, a filamentous fungus with multifaceted ecological and economic roles.

Mengmeng Yang; Feiyu Yan; Qingzhou Ma; Yanling Zhang; Bingye Yang; Yashuang Guo; Meng Zhang; Taibo Liang; Chao Xu

doi:10.1038/s41597-026-07525-4

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Chromosome-level de novo assembly of the nuclear and mitochondrial genomes of Arcopilus aureus, a filamentous fungus with multifaceted ecological and economic roles.

Mengmeng Yang·Feiyu Yan·Qingzhou Ma·Yanling Zhang·Bingye Yang·Yashuang Guo·Meng Zhang·Taibo Liang·Chao Xu

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Abstract

The filamentous fungus Arcopilus aureus (Sordariale: Chaetomiaceae) is notable for its multi-domain significance across agriculture, medicine, and industry. In this study, we generated a chromosome-level nuclear genome and a complete circular mitogenome for A. aureus by integrating data from next-generation sequencing, PacBio HiFi, and Hi-C technologies. The final nuclear genome assembly spans 33.77 Mb (GC content: 57.67%), and was organized into seven chromosomal-sized scaffolds (only one gap) with an N50 size of 5.09 Mb and BUSCO completeness of 95.91%. A total of 10,282 protein-coding genes, 228 non-coding RNAs, and ~1.77 Mb of repetitive elements were predicted in the nuclear genome. By contrast, the mitogenome of A. aureus is 33,820 bp in length, with a GC content of 25.96%. It harbors 15 typical mitochondrial protein-coding genes, one unidentified ORF, two rRNAs (small subunit rns and large subunit rnl), and 28 tRNAs. This high-quality genome assembly provides a valuable resource for understanding the ecology, genetics, and evolution of A. aureus, which facilitates elucidating its mechanisms of biocontrol, infection, and metabolite synthesis.

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Genome, Mitochondrial Genome, Fungal Chromosomes, Fungal Journal Article Dataset

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Mengmeng Yang, Feiyu Yan, Qingzhou Ma, Yanling Zhang, Bingye Yang, Yashuang Guo, Meng Zhang, Taibo Liang, Chao Xu. 2026-06-02. Chromosome-level de novo assembly of the nuclear and mitochondrial genomes of Arcopilus aureus, a filamentous fungus with multifaceted ecological and economic roles.. https://doi.org/10.1038/s41597-026-07525-4

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