Minicolumn detection method applied to almonds: collaborative study.

G I Stanley; V P DiProssimo; A C Koontz

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PubMed · 422495

Minicolumn detection method applied to almonds: collaborative study.

G I Stanley·V P DiProssimo·A C Koontz

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Abstract

The minicolumn screening method for aflatoxins was collaboratively tested on naturally contaminated almonds. The nuts were extracted, and the extract was cleaned up and applied to a Velasco-type minicolumn. This permits the detection of total aflatoxins (B1, B2, G1, G2) as a fluorescent band on the Florisil layer of the column. The results of 20 collaborators are presented. Samples containing 0, 2, 5, 10, and 25 ng aflatoxin/g were analyzed. Ninety-six per cent of the samples containing 5--25 ng total aflatoxins/g and 83% of the negative samples were correctly identified. The method has been adopted as official first action for detection of total aflatoxin levels of greater than or equal to 5 ng/g.

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G I Stanley, V P DiProssimo, A C Koontz. 1979. Minicolumn detection method applied to almonds: collaborative study.. https://pubmed.ncbi.nlm.nih.gov/422495/

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