Rate-limiting steps in metabolic pathways.

R Rognstad

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PubMed · 422559

Rate-limiting steps in metabolic pathways.

R Rognstad

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Abstract

A method is proposed to detect whether a given enzyme catalyzes a rate-limiting step in a metabolic pathway. With the use of a range of concentrations of specific inhibitors of an enzyme, the finding of a biphasic response with an initial null effect indicates the non-rate-limiting nature of the enzyme. With this method, phosphoenolpyruvate carboxykinase is indicated to catalyze a rate-limiting step in lactate gluconeogenesis in hepatocytes from fasted rats.

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Aminooxyacetic Acid Animals Cycloserine Dose-Response Relationship, Drug Gluconeogenesis Kinetics Lactates Lipids Liver Male Phosphoenolpyruvate Carboxykinase (GTP)Picolinic Acids Rats Sulfhydryl Compounds Journal Article Research Support, U.S. Gov't, P.H.S.

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R Rognstad. 1979-03-25. Rate-limiting steps in metabolic pathways.. https://pubmed.ncbi.nlm.nih.gov/422559/

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