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IVM rescue: Effect of growth hormone supplementation combined to autologous cumulus co-culture on GV oocyte maturation and competency.

Chaimaa Hilali·Noureddine Louanjli·Wassym Senhaji Rhazi·Mohammed Ennaji·Mohamed Kettani Halabi·Nouama Bouanani·El Mehdi Hissane·Mohamed Zarquaoui·Rosalie Cabry·Fatima Azzahra Lahlou·Moncef Benkhalifa
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Abstract

OBJECTIVE: IVM rescue is based on the in vitro maturation of mainly Germinal Vesicle (GV) oocytes collected from stimulated cycles. The objective was to investigate the effects of growth hormone (GH) and autologous cumulus cells co culture (CC) on oocyte meiosis resumption and maturation after 32 h post cumulus denudation, in order to obtain additional embryos for the couple as a rescue system to increase the changes of cumulative pregnancy. MATERIAL AND METHODS: Our study concerned 300 patients who underwent ICSI cycles, during which a total of 1940 cumulus-complex-oocytes were retrieved, giving 1260 metaphase II stage (MII), 200 at the metaphase I stage, and 480 at the Germinal Vesicle (GV) stage. Mature oocytes were microinjected on the same day of retrieval. Immature GV oocytes were divided into four groups, with the first undergoing in vitro maturation (IVM) without cumulus cells (group 1) and the second undergoing IVM with CC (group 2), the third undergoing IVM without CC and with GH (group 3), the fourth undergoing IVM with CC and with GH (group 4). After 32 h of IVM, the matured oocytes, underwent microinjection, followed by embryonic development monitoring. RESULTS: When comparing the IVM outcomes, we observed a significant increase in oocyte maturation, fertilization rates and the percentage of 8-cell embryos on day 3 across the different study groups (p < 0.001) (Figs. 2-5). Furthermore, all study groups (1-4) exhibited notably blastulation rates, with group 3 demonstrating the most promising clinical outcomes. A preliminary pregnancy rate of approximately 20% was recorded in group 3, suggesting a potential improvement in the developmental competence of oocytes matured under specific conditions. CONCLUSION: The IVM rescue of germinal vesicle oocyte could serve as an additional strategy to increase the chance getting extra embryos to patients. Autologous cumulus cells co-culture combined to GH supplementation to IVM media, appear to play a crucial role to enhance successful meiosis resumption, oocyte maturation and competency to support embryos development when the injected spermatozoa is not carrier of severe genome and epigenomic decays.

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HumansFemaleIn Vitro Oocyte Maturation TechniquesCumulus CellsCoculture TechniquesOocytesPregnancyAdultSperm Injections, IntracytoplasmicGrowth HormonePregnancy RateCumulus cell co culture and growth hormoneEmbryological outcomesFertilization rateIn vitro maturationJournal Article

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Chaimaa Hilali, Noureddine Louanjli, Wassym Senhaji Rhazi, Mohammed Ennaji, Mohamed Kettani Halabi, Nouama Bouanani, El Mehdi Hissane, Mohamed Zarquaoui, Rosalie Cabry, Fatima Azzahra Lahlou, Moncef Benkhalifa. 2026-06-23. IVM rescue: Effect of growth hormone supplementation combined to autologous cumulus co-culture on GV oocyte maturation and competency.. https://doi.org/10.1016/j.jogoh.2026.103237

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