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Occurrence of blaOXA-72 in a clinical isolate of carbapenem-resistant Acinetobacter pittii ST206 in Japan.

Taku Yamane·Tomohiro Denda·Tatsuya Natori·Tomu Kamijo·Ayaka Hachiro·Takeshi Uehara·Yukiko Nagano·Noriyuki Nagano
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Abstract

The development of carbapenem resistance in Acinetobacter spp., which are recognized as significant opportunistic pathogens, is of critical importance as it poses challenges to therapy and the control of healthcare-associated infections in clinical settings. This study investigated the genetic characteristics of a carbapenem-resistant A. pittii clinical isolate from a university hospital using whole-genome sequencing. The A. pittii strain SU8507, which was detected in the abdominal drainage fluid of a patient, exhibited resistance to imipenem (MIC: 128 μg/mL) and meropenem (MIC: 64 μg/mL) and produced positive results by the CIMTris method. A. pittii SU8507, belonging to ST206, harbored the blaOXA-72 and new variants of intrinsic blaOXA-213-like gene blaOXA-1222, which lacks an upstream insertion sequence element, and blaADC-1-like gene blaADC-343. The blaOXA-72 gene, flanked by XerC/XerD-like recombination sites, was located on a plasmid pSU8507, sized at 10,913 bp, carrying 13 predicted protein-coding genes. Complete pSU8507 containing mobA, repB, and the yoeB-yefM toxin-antitoxin genes, showed 98.9% nucleotide sequence identity and 75% coverage with plasmid pA2702 of the A. baylyi strain A2702, but a low BLAST MAX score. SU8507 harbored virulence genes involved in biofilm formation, types II and VI secretion systems, type IV pilus system, and serum resistance. This study describes the first isolation of an OXA-72-producing, carbapenem-resistant A. pittii clinical isolate in Japan. Given that the isolation rate of carbapenem-resistant Acinetobacter spp. Remains low in Japan, it is crucial to expand the scope of rapid, accurate carbapenemase detection to include not only A. baumannii, but also non-baumannii Acinetobacter spp.

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HumansAcinetobacterAcinetobacter InfectionsAnti-Bacterial AgentsBacterial Proteinsbeta-Lactam Resistancebeta-LactamasesCarbapenemsImipenemJapanMeropenemMicrobial Sensitivity TestsPlasmidsWhole Genome SequencingAcinetobacter pittiiCarbapenemaseOXA-72PlasmidJournal Article

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Taku Yamane, Tomohiro Denda, Tatsuya Natori, Tomu Kamijo, Ayaka Hachiro, Takeshi Uehara, Yukiko Nagano, Noriyuki Nagano. 2026-07-17. Occurrence of blaOXA-72 in a clinical isolate of carbapenem-resistant Acinetobacter pittii ST206 in Japan.. https://doi.org/10.1016/j.jiac.2026.103036

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