Comparison of endothelin-1 levels in plasma from human coronary arteries measured by enzyme linked immunosorbent assay and Olink high-throughput proteomics platform.

Majid Anwar; Rhoda E Kuc; Catherine Bullock; George R Abraham; Janet J Maguire; Stephen P Hoole; Diane Proudfoot; Anthony P Davenport

doi:10.1139/cjpp-2026-0112

PubMed · 42628109

Comparison of endothelin-1 levels in plasma from human coronary arteries measured by enzyme linked immunosorbent assay and Olink high-throughput proteomics platform.

Majid Anwar·Rhoda E Kuc·Catherine Bullock·George R Abraham·Janet J Maguire·Stephen P Hoole·Diane Proudfoot·Anthony P Davenport

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Abstract

Endothelin-1 (ET-1) antagonists are increasingly being approved for new treatments for cardiovascular disease, where elevated ET-1 levels contribute to increased vasoconstriction. Further therapeutic targets, including coronary artery disease, are under investigation. The Olink Explore 3072 Proximity Extension Assay platform enables multiplexed high-throughput measurement of ~3000 plasma proteins, from minimal (≤6 µL) sample volumes. However, it is not known if the two oligonucleotide-tagged antibodies raised against preproET-11-212, used in this Olink assay, specifically measure biologically active ET-1 or the other inactive EDN1-encoded peptides, also secreted by human endothelial cells. Paired plasma samples from 29 patients with coronary artery disease were obtained, using a specialised intra-coronary sampling catheter, designed to obtain site specific biochemical information from within coronary arteries. We compared ET-1 concentrations measured with an ET-1 specific ELISA, demonstrated to have no cross-reactivity with other EDN1-encoded peptides versus values obtained using Olink Explore platform. Olink-measured ET-1 correlated significantly with ELISA-derived ET-1 levels (r = 0.53, p = 0.003), and Olink values predicted ELISA results. Olink ET-1 concentrations also correlated with ETB receptor levels (r = 0.40, p < 0.05). These findings indicate that the Olink Explore platform can detect relative changes in biologically active ET-1, supporting its use as a biomarker tool in clinical and translational studies.

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Humans Endothelin-1 Proteomics Coronary Vessels Enzyme-Linked Immunosorbent Assay Male Coronary Artery Disease Female High-Throughput Screening Assays Middle Aged ELISA Olink Olink Explore 3072 coronary artery disease endothelin-1 proteomics Journal Article Comparative Study

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Majid Anwar, Rhoda E Kuc, Catherine Bullock, George R Abraham, Janet J Maguire, Stephen P Hoole, Diane Proudfoot, Anthony P Davenport. 2026-01-01. Comparison of endothelin-1 levels in plasma from human coronary arteries measured by enzyme linked immunosorbent assay and Olink high-throughput proteomics platform.. https://doi.org/10.1139/cjpp-2026-0112

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