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Restoration contexts shape the bacterial and fungal soil communities in desertification hotspots in the Brazilian semiarid region.

Larissa Macelle de Paulo Barbosa·Davila Esmelinda Oliveira Silva·Sabrina Hermelindo Ventura·Mayanna Karlla Lima Costa·Sandra Mara Barbosa Rocha·Jhoice Ferreira Borges·Romario Martins Costa·Erika Valente de Medeiros·Arthur Prudêncio de Araujo Pereira·Lucas William Mendes·Joana Falcão Salles·Ademir Sergio Ferreira Araujo
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Abstract

Desertification in the Brazilian semiarid has compromised ecosystem functionality, impacting soil microbial biodiversity. Thus, restoration strategies have been implemented, aiming to mitigate the negative impacts. However, little is known about their effects on soil microbial communities. In this study, we hypothesized that the two restoration contexts would promote distinct trajectories of soil microbial community recovery. We evaluated 36 soil samples collected from two desertification hotspots in the Brazilian semiarid, representing active (Gilbués) and passive (Irauçuba) restoration contexts. Soil DNA was extracted and subjected to 16S and ITS amplicon sequencing to characterize bacterial and fungal communities, respectively. Community differences were assessed using alpha-diversity metrics, redundancy analysis (RDA), and PERMANOVA. The results showed that within Gilbués (active restoration), bacterial and fungal community composition differed among soils under desertification and restoration. In Irauçuba (passive restoration), only native soils differed from both soils under desertification and restoration. Proteobacteria, Actinobacteriota, and Firmicutes (bacteria), and Ascomycota and Basidiomycota (fungi), were the dominant phyla in both hotspots. Bacterial and fungal communities showed distinct taxonomic patterns among native, degraded, and restored soils within each restoration context. Niche occupancy patterns also differed between restoration contexts. In conclusion, the two hotspots followed contrasting microbial recovery trajectories, demonstrating that restoration responses are context-dependent and vary according to the microbial groups, rather than supporting the universal superiority of one restoration strategy over the other.

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Soil MicrobiologyBrazilBacteriaFungiBiodiversitySoilConservation of Natural ResourcesDesert ClimateEcosystemDegradationMicrobial communityNiche occupancySoil restorationJournal Article

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Larissa Macelle de Paulo Barbosa, Davila Esmelinda Oliveira Silva, Sabrina Hermelindo Ventura, Mayanna Karlla Lima Costa, Sandra Mara Barbosa Rocha, Jhoice Ferreira Borges, Romario Martins Costa, Erika Valente de Medeiros, Arthur Prudêncio de Araujo Pereira, Lucas William Mendes, Joana Falcão Salles, Ademir Sergio Ferreira Araujo. 2026-08-29. Restoration contexts shape the bacterial and fungal soil communities in desertification hotspots in the Brazilian semiarid region.. https://doi.org/10.1016/j.jenvman.2026.130831

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