PubMed · 42687106
Testing for Bacteria and Fungi in Cells and Ancillary Reagents-Probe-Based Quantitative PCR Assays.
Abstract
This document specifies the technical elements for bacteria and fungi detection by PCR assay which enables rapid, broad-spectrum detection of bacteria and fungi with high sensitivity. Qualitative judgement is based on LOD. Key detection points include efficient nucleic acid extraction, broad-spectrum primer-probe design, high amplification efficiency and minimised background interference.
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Jiani Cao, Ranran Xu, Boqiang Fu, Jie Hao, Lei Wang, Aijin Ma, Qiang Wei, Hongling Zhao, Chunming Rao, Qiyuan Li, Tao Na, Shijun Hu, Lihua Zhou, Weiqi Zhang, Qubo Chen, Siming Sun, Zengshu Liu, Shuaishuai Niu, Tingting Zhang, Yuanyuan Zhang, Yidi Gan, Shuyan Wang, Glyn N Stacey, Jun Wei, Tongbiao Zhao, Yu Alex Zhang. 2026-09-02. Testing for Bacteria and Fungi in Cells and Ancillary Reagents-Probe-Based Quantitative PCR Assays.. https://doi.org/10.1111/cpr.70270
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