A guide to selecting high-performing antibodies for TMEM175 (UniProt ID: Q9BSA9) for use in western blot, immunoprecipitation, and immunofluorescence.

Vera Ru&#xed;z Mole&#xf3;n; Charles Alende; Maryam Fothouhi; Sara Gonz&#xe1;lez Bol&#xed;var; Riham Ayoubi; Vincent Francis; Peter S McPherson; Carl Laflamme

doi:10.12688/f1000research.182354.1

PubMed · 42728995

A guide to selecting high-performing antibodies for TMEM175 (UniProt ID: Q9BSA9) for use in western blot, immunoprecipitation, and immunofluorescence.

Vera Ruíz Moleón·Charles Alende·Maryam Fothouhi·Sara González Bolívar·Riham Ayoubi·Vincent Francis·Peter S McPherson·Carl Laflamme

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Abstract

TMEM175 is the pore-forming subunit of a lysosomal K+ channel complex that regulates lysosomal pH stability and membrane potential. To further investigate its cellular functions and implications in neurodegenerative diseases, antibody reagents are needed. Here we have characterized six TMEM175 commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.

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Humans Immunoprecipitation Blotting, Western Fluorescent Antibody Technique Antibodies Potassium Channels Endosomal/lysosomal proton channel TMEM175 Potassium channel TMEM175 Q9BSA9 TMEM175 Transmembrane protein 175 antibody characterization antibody validation immunofluorescence immunoprecipitation western blot Journal Article

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Vera Ruíz Moleón, Charles Alende, Maryam Fothouhi, Sara González Bolívar, Riham Ayoubi, Vincent Francis, Peter S McPherson, Carl Laflamme. 2026-06-16. A guide to selecting high-performing antibodies for TMEM175 (UniProt ID: Q9BSA9) for use in western blot, immunoprecipitation, and immunofluorescence.. https://doi.org/10.12688/f1000research.182354.1

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