Reproduction on the Rocks: Life History of a Freshwater Macrobioeroding Bivalve.

Abstract

Macrobioerosion, the excavation and removal of consolidated mineral substrates by macrofauna, is well established in marine systems, where macrobioeroders drive carbonate cycling, sediment production and habitat formation. In freshwater ecosystems, however, it has been documented in only a small number of invertebrate taxa and remains a poorly resolved ecological process. Among these, the teredinid shipworm Lithoredo abatanica represents a remarkable departure from the wood-boring ecology of its family, having evolved to excavate and ingest limestone in fresh water. Despite this remarkable ecological transition, its reproductive biology and life history remain unknown. Here, we investigate the reproductive mode and life history strategy of this species using population size structure, in&#xa0;situ observations of siphonal morphology, and sperm morphometrics. We show that L.&#x2009;abatanica reaches exceptional dimensions, with measured body lengths exceeding 100&#x2009;mm, one intact empty burrow exceeding 200&#x2009;mm and in-water observations indicating burrows possibly exceeding 500&#x2009;mm, establishing it as the largest known freshwater macrobioeroder. Its large size and dense aggregations indicate considerable capacity for local rock breakdown and habitat modification within the Abatan River system (Bohol, Philippines). Recently settled juveniles (<&#x2009;10&#x2009;mm) alongside reproductively mature individuals indicate ongoing recruitment. The morphology of the siphons and calcareous tube appears to preclude direct sperm transfer via pseudocopulation, while sperm morphometrics are consistent with external fertilisation. Together, these findings indicate that L.&#x2009;abatanica reproduces via broadcast spawning with external fertilisation and likely possesses a planktotrophic larval phase. This raises a fundamental question: how does a broadcast-spawning species with planktotrophic larvae maintain populations up to 15&#x2009;km upstream in a flowing freshwater river subject to persistent downstream advection? By resolving the life history of the largest known freshwater macrobioeroder, this study provides critical insight into the persistence, dispersal and ecological role of a globally unique riverine ecosystem engineer.