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The relationship between stem cell seeding efficiency and position in cell cycle.

F C Monette·J B DeMello
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Abstract

The seeding efficiency of colony-forming cells from normal, regenerating and velocity-sedimented cycling and non-cycling narrow preparations was compared. Colony-forming cells in cycle were found to exhibit a 50% reduction in splenic seeding when compared to normal marrow or sedimented non-cycling cells. The results of this study indicate that the spleen colony assay underestimates the total number of colony-forming cells by a fraction which is directly related to the number of cells in cycle.

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AnimalsBone MarrowCell CycleColony-Forming Units AssayFemaleHematopoietic Stem CellsMiceRegenerationSpleenComparative StudyJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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F C Monette, J B DeMello. 1979. The relationship between stem cell seeding efficiency and position in cell cycle.. https://doi.org/10.1111/j.1365-2184.1979.tb00123.x

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