Diphtheria toxin subunit active in vitro.

R J Collier; H A Cole

doi:10.1126/science.164.3884.1179

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PubMed · 4305968

Diphtheria toxin subunit active in vitro.

R J Collier·H A Cole

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Abstract

Exposure of diphtheria toxin to dithiothreitol (and similar thiols) resulted in a subunit which was active in catalyzing the adenosine diphosphateribosylation of mammalian aminoacyl-transferase II in the presence of nicotinamide adenine dinucleotide. At the same time there was a marked increase in total ADP-ribosylation activity. A molecule which was apparently identical to the derived subunit in size and activity was detected in partially purified preparations of toxin.

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Adenine Nucleotides Catalysis Centrifugation, Density Gradient Chromatography, Gel Diphtheria Toxin NAD Sulfhydryl Compounds Journal Article

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R J Collier, H A Cole. 1969-06-06. Diphtheria toxin subunit active in vitro.. https://doi.org/10.1126/science.164.3884.1179

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