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Artificial substrates for prenyltransferase.

G Popják·J L Rabinowitz·J M Baron
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Abstract

Four out of 16 new allylic pyrophosphates synthesized were found to be artificial substrates for liver prenyltransferase (EC 2.5.1.1). These were the trans-and the cis-3-ethyl-3-methylallyl, the 3,3-diethylallyl and the (mixture of cis and trans) 3-methyl-3-n-propylallyl pyrophosphates. The products synthesized from these substrates and isopentenyl pyrophosphate were the appropriate homo- and bishomo-farnesyl pyrophosphates. Substitution of 3,3-dimethylallyl pyrophosphate at C-2 with a methyl group destroyed its reactivity with the enzyme. Neither the unsubstituted allyl pyrophosphate nor the cis- or trans-3-methylallyl pyrophosphate could be condensed with isopentenyl pyrophosphate. Thus the simplest allylic substrate for prenyltransferase is 3,3-dimethylallyl pyrophosphate.

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AnimalsChromatography, GasDiphosphatesFarnesolLiverSpectrum AnalysisSwineTransferasesJournal Article

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G Popják, J L Rabinowitz, J M Baron. 1969. Artificial substrates for prenyltransferase.. https://doi.org/10.1042/bj1130861

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