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PubMed · 43205

Spectrofluorometric assay of gentamicin in serum.

Z M Habbal
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Abstract

A spectrofluorometric method has been developed for the assay of gentamicin in serum. The fluorogen of this reaction is the Zn2+ chelate of N-pyridoxylidene gentamicin. The ratio of gentamicin to Zin is 1.0. Interfering compounds in serum are removed by chromatography on Bio-Rex 70. Recovery of added gentamicin is between 90 and 100%. Determination of gentamicin levels in patient sera correlate well with those done by bioassay. This assay is relatively fast (about 1 h), and requires 1.0 ml of serum. It also can be applied to the determination of other deoxystreptamine antibiotics such as kanamycin and tobramycin.

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Biological AssayGentamicinsHumansHydrogen-Ion ConcentrationSolubilitySpectrometry, FluorescenceZincJournal Article

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BibTeXRIS

Z M Habbal. 1979-07-16. Spectrofluorometric assay of gentamicin in serum.. https://doi.org/10.1016/0009-8981(79)90372-3

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In vivo recovery of factor VIII: a comparison of one-stage and two-stage assay methods.

The recovery and half-life of VIII:C in the plasma of severely haemophilic patients was measured by one-stage and two-stage assays after injection of two Factor VIII concentrates (Hemofil, Hyland and Fraction I-O, Kabi). Plasma volumes were measured with an Evans' Blue technique, and both concentrates and post-infusion samples were measured against the same plasma standard. There was a highly significant difference in recoveries estimated by the two assay methods. The one-stage assays gave the most consistent results, in that the average recovery was 100%, whereas the two-stage assays gave only about 80% of the value expected from in vitro assays. There was no differences in recoveries between the two concentrates. The two-stage assays gave a slightly shorter half-life than the one-stage assays, and the half-life of Hemofil was also shorter than that of Fraction I-O.

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