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PubMed · 434841

The Mg insertion step in chlorophyll biosynthesis.

P A Castelfranco·J D Weinstein·S Schwarcz·A D Pardo·B E Wezelman
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Aminolevulinic AcidChlorophyllChloroplastsGlutamatesKineticsMagnesiumPlantsPorphyrinsProtoporphyrinsSpectrometry, FluorescenceJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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P A Castelfranco, J D Weinstein, S Schwarcz, A D Pardo, B E Wezelman. 1979. The Mg insertion step in chlorophyll biosynthesis.. https://doi.org/10.1016/0003-9861(79)90130-9

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PubMed1979

Determination of 5-aminolaevulinic acid and porphobilinogen by high-performance liquid chromatography.

A strong anion-exchange column coupled to a strong cation-exchange column with acetate buffer as eluent or reversed-phase ion-pair chromatography on octadecylsilica with methanol-water containing 1-heptanesulphonic acid as the mobile phase is described for the simultaneous separation of 5-aminolaevulinic acid and porphobilinogen. The separation is applied to the development of a fast and simple method for determining the activity of the enzyme aminolaevulinic acid dehydrase in human erythrocytes. 5-Aminolaevulinic acid is used as the enzyme substrate and the enzyme activity is expressed as micromoles of porphobilinogen formed per ml of erythrocytes in 1 h at 38 degrees. 5-Aminolaevulinic acid and porphobilinogen can also be separated from the urine of prophyric patients but the UV detector has insufficient sensitivity for the determination of 5-aminolaevulinic acid.

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