PubMed · 436621
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R Ammann. 1979-04-13. [Fluorescein-dilaurate test].. https://pubmed.ncbi.nlm.nih.gov/436621/
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The reassociation of antibody (IgG)-derived heavy (gamma) and light (L) chains was studied by utilizing two specifically localized fluorescent probes. L chains labeled in either the variable domain with fluorescein (specific antigen) or the constant domain with N-(iodoacetamidoethyl)-5-naphthylamine-1-sulfonic acid were prepared. Reassociation of these chains with isolated, unlabeled, gamma chains was monitored by following an increase in the fluorescence polarization of fluorophores at 4 degrees C. Results obtained indicate that the reassociation reaction could be resolved into two distinct steps; the primary interaction of the reactants occurring rapidly, followed by a slower secondary phase possibly representing conformational changes in the variable domains of the reactants after reassociation.
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The fluorescein diacetate-ethidium bromide (FDA-EB) fluorescence method, primarily used to determine viability of mammalian cells, was applied to several fungi species. Living fungi cells produced fluorochromasia, i.e., an intracellular accumulation of fluorescein which could be easily visualized as a green color under the U.V. microscope. Dead cells showed a red bright color due to ethidium bromide penetration. The FDA-EB test can be successfully employed to assay yeast and yeast like cells viability since a good correlation was observed between this assay and the colony count technique. The main advantages of FDA-EB test are its speed, high sensitivity and simplicity.