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PubMed · 441727

Flow cytometry: a high-resolution instrument for everyone.

H B Steen·T Lindmo
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Abstract

A new flow configuration for flow cytometry has been devised in which a flat, laminar stream of water, containing the stained cells in a narrow sector, is formed on a microscope cover slip by a pressurized jet of water directed onto the glass at low angle. The stream of cells is viewed by means of a fluorescence microscope with incident illumination and a pulse photometer. Coupled to a multichannel pulse height analyzer, the instrument constitutes a stable and easy-to-operate flow cytometer with a resolution equal to or better than a coefficient of variance of 1.4 percent in measurements of cellular DNA.

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AnimalsCell SeparationDNAMicroscopy, FluorescenceRatsThymus GlandJournal Article

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H B Steen, T Lindmo. 1979-04-27. Flow cytometry: a high-resolution instrument for everyone.. https://doi.org/10.1126/science.441727

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