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PubMed · 4469696

Decrease in free cystine content of cultured cystinotic fibroblasts by ascorbic acid.

W A Kroll·J A Schneider
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Abstract

The 100-fold increase in free cystine content characteristic of cultured skin fibroblasts from patients with nephropathic cystinosis was decreased more than 50 percent by addition of L-ascorbic acid to the culture medium at concentrations of 0.29 to 2.9 millimolar. Fresh ascorbic acid must be added to the culture medium daily to produce a progressive decrease of the free cystine content of the cells over a 3-day period. Upon removal of ascorbic acid from the medium, the free cystine content returns to its initial value.

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Ascorbic AcidCells, CulturedCystineCystinosisDithiothreitolJournal Article

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W A Kroll, J A Schneider. 1974-12-13. Decrease in free cystine content of cultured cystinotic fibroblasts by ascorbic acid.. https://doi.org/10.1126/science.186.4168.1040

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