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Fluorometric assay of the arylsulphatases in human urine.

B Hultberg
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Abstract

A method is described which differentiates arylsulphatase A and arylsulphatase B in human urine. 4-Methylumbelliferyl sulphate serves as the substrate, and silver ions are used to inhibit arylsulphatase A activity. Using fresh urine it is possible to obtain separate values for arylsulphatase A and B when both are present, thus providing a diagnostic test for metachromatic leucodystrophy.

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Cerebroside-SulfataseChondro-4-SulfataseChromatography, Ion ExchangeClinical Enzyme TestsHumansHydrogen-Ion ConcentrationLeukodystrophy, MetachromaticSpectrometry, FluorescenceSulfatasesJournal Article

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BibTeXRIS

B Hultberg. 1979. Fluorometric assay of the arylsulphatases in human urine.. https://doi.org/10.1515/cclm.1979.17.12.795

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PubMed1979

Arylsulphatases A and B in human diploid fibroblasts: differential assay with 4-methylumbelliferylsulphate and AgNO3.

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The activity of arylsulfatase A and B on tyrosine O-sulfates.

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