Messenger selection by bacterial ribosomes.

S Leffler; W Szer

doi:10.1073/pnas.70.8.2364

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PubMed · 4525171

Messenger selection by bacterial ribosomes.

S Leffler·W Szer

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Abstract

The counterpart of Escherichia coli initiation factor 3(IF-3) was isolated from Caulobacter crescentus, purified to homogeneity, and used in comparative studies on in vitro translation of RNA from the C. crescentus RNA phage Cb5 and of coliphage MS2 RNA. The two phage RNAs are similar in physical properties and analogous in genetic content. The factor, C-IF-3, substitutes for E. coli IF-3 and promotes correct translation of MS2 RNA by E. coli ribosomes. Conversely, E. coli IF-3 substitutes for C-IF-3 in translation of Cb5 RNA by C. crescentus ribosomes. However, each phage RNA could be translated only by host ribosomes or by mixed ribosomes containing the host 30S subunit. C-IF-3 dissociates C. crescentus and E. coli 70S ribosomes into subunits. It binds phage, ribosomal, and, less efficiently, transfer RNA.

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Amino Acids Bacteria Bacteriophages Carbon Isotopes Coliphages Electrophoresis, Polyacrylamide Gel Peptide Initiation Factors Protein Biosynthesis RNA, Viral Ribosomes Species Specificity Tritium Viral Proteins Journal Article

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S Leffler, W Szer. 1973. Messenger selection by bacterial ribosomes.. https://doi.org/10.1073/pnas.70.8.2364

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