[Primary structure of abnormal E-like hemoglobin].

V A Spivak; T P Molchanova; N V Ermakov; Iu N Tokarev; R F Gar'kavtseva

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PubMed · 454712

[Primary structure of abnormal E-like hemoglobin].

V A Spivak·T P Molchanova·N V Ermakov·Iu N Tokarev·R F Gar'kavtseva

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Abstract

An E-like abnormal hemoglobin was detected in a hematological patient and one of the members of her family. The composition of blood hemolyzates was characterized using acetate cellulose electrophoresis. The abnormal beta-chain was isolated by ion-exchange chromatography of total globin on CM-cellulose. Using peptide mapping of the abnormal beta-chain trypsin hydrolysates, it was shown that the amino acid substitution occurs in peptide beta T3. The amino acid analysis and determination of the abnormal fragment C-terminal amino acid allowed to establish the locus and type of this substitution. The first case of hemoglobin E(alpha 2 beta 2 26Glu leads to Lys) identification on the territory of the USSR is reported.

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Amino Acid Sequence Glutamates Hemoglobin E Hemoglobins, Abnormal Humans Lysine Macromolecular Substances Peptide Fragments English Abstract Journal Article

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V A Spivak, T P Molchanova, N V Ermakov, Iu N Tokarev, R F Gar'kavtseva. 1979. [Primary structure of abnormal E-like hemoglobin].. https://pubmed.ncbi.nlm.nih.gov/454712/

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