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A sensitive radiometric assay for cysteic acid decarboxylase activity in crude enzyme preparations of rat liver and brain.

C F Yam·G Tunnicliff·T T Ngo·A Barbeau
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Abstract

1. A method is described for the synthesis of L-[U-14C]cysteic acid from L-[U-14C] cysteine hydrochloride and for its subsequent utilisation as a substrate for cysteic acid decarboxylase activity in liver and brain. 2. The enzyme determination relies on the entrapment of radio-labelled carbon dioxide in Hyamine hydroxide. 3. The assay is sensitive, reliable and convenient and is particularly suitable for measuring the activity of the decarboxylase in crude enzyme preparations.

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AnimalsBrainCarboxy-LyasesCysteic AcidHydrogen-Ion ConcentrationLiverMaleRadiometryRatsRats, Inbred StrainsSubstrate SpecificityJournal ArticleResearch Support, Non-U.S. Gov't

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C F Yam, G Tunnicliff, T T Ngo, A Barbeau. 1977. A sensitive radiometric assay for cysteic acid decarboxylase activity in crude enzyme preparations of rat liver and brain.. https://doi.org/10.1016/0305-0491(77)90136-5

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