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PubMed · 4552891

Batch- and continuous-culture transients for two substrate systems.

C N Standing·A G Fredrickson·H M Tsuchiya
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Abstract

Batch growth of Escherichia coli in the presence of equal initial concentrations of glucose and a secondary substrate (xylose) is characterized by sequential utilization of the substrates, whereas continuous-culture systems with equal concentrations of the two substrates in the feed are characterized by complete utilization of both substrates at both high and low dilution rates. Such systems at steady state at a low dilution rate, when suddenly shifted to a higher dilution rate, experience a transient drop in population density accompanied by accumulation of the secondary substrate but virtually no accumulation of glucose. Systems at steady state with 200 mg of glucose per liter were found to undergo a transient population decrease and eventual recovery when switched to feed containing 200 mg of a secondary substrate per liter.

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Bacteriological TechniquesCulture MediaEscherichia coliGalactoseGlucoseXyloseJournal Article

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C N Standing, A G Fredrickson, H M Tsuchiya. 1972. Batch- and continuous-culture transients for two substrate systems.. https://doi.org/10.1128/am.23.2.354-359.1972

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