PubMed · 4563438
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P Borst. 1972. Mitochondrial nucleic acids.. https://doi.org/10.1146/annurev.bi.41.070172.002001
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Lucigenin (bis-N-methylacridinium) (Luc2+) has frequently been used for the luminescent detection of O2-. In fact, the pathway leading to this luminescence requires univalent reduction of Luc2+ to LucH.+ followed by formation of an unstable dioxetane by reaction of LucH.+ with O2-. It is now shown that LucH.+ rapidly autooxidizes and so produces O2-. Luc2+ can thus mediate O2- production in systems, such as glucose plus glucose oxidase, in which there is ordinarily no O2- production. Luc2+ luminescence can thus be used as the basis for assaying superoxide dismutase activity but should not be used for measuring, or even detecting, O2-.
PURPOSE: We attempted to determine reactive oxygen species production by the spermatozoa of patients with idiopathic infertility and healthy donors, and observe whether increased production was due to decreased seminal plasma reactive oxygen species scavengers. MATERIALS AND METHODS: Reactive oxygen species production by spermatozoa and seminal plasma antioxidants was assayed in 18 patients with idiopathic infertility and 10 controls. Reactive oxygen species formation and seminal plasma antioxidants were measured by luminol and lucigenin dependent chemoluminescence, and enzymatic methods, respectively. RESULTS: Higher reactive oxygen species production was observed in 16 of the 18 patients (88.8%, p < 0.0001 versus controls). Seminal plasma superoxide dismutase, catalase, glutathione peroxidase and total sulfhydryl group levels in infertile patients were significantly lower than in controls. CONCLUSIONS: Decreased seminal plasma antioxidant activity and increased reactive oxygen species production can be responsible for idiopathic male infertility.
Seven 1,2-dihydroxy-1,2-dihydroacronycine and 1,2-dihydroxy-1,2-dihydro-6-demethoxyacronycine esters and diesters were synthesized via osmic oxidation of acronycine or 6-demethoxyacronycine followed by acylation. The 6-demethoxyacronycine derivatives were found to be inactive, whereas in contrast, all of the acronycine derivatives were more potent than acronycine itself when tested against L1210 cells in vitro. Four selected acronycine derivatives (17,19, 21, and 22) were evaluated in vivo against murine P388 leukemia and colon 38 adenocarcinoma implanted in mice. All compounds were markedly active against P388 at doses 4-16-fold lower than acronycine itself. Against the colon 38 adenocarcinoma, the three compounds 17, 21, and 22 were highly efficient. 1,2-Diacetoxy-1,2-dihydroacronycine (17) was the most active, all the treated mice being tumor-free on day 23.