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Recipient gene duplication during generalized transduction.

M Stodolsky
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Abstract

An Hfr13 Delta(proA-lac) deletion recipient, -Delta(proA-lac)-F-purE(+)-, has been utilized in a study of the origins of duplications formed during chromosome fragment integration. Among the Pro(-)Lac(+) transductants, some have duplications spanning the F locus. These transductants are, or segregate, strains with F' episomes carrying genes of the duplication. Some of the duplications include purE(+), a gene which is not coinherited with lac(+) during bacteriophage P1-mediated transduction. Thus recipient genes have been duplicated during recombinant formation. Crossing-over models including replication steps provide a basis for explaining the duplication process.

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Chromosome AberrationsChromosome MappingChromosomes, BacterialColiphagesConjugation, GeneticCrossing Over, GeneticEscherichia coliExtrachromosomal InheritanceGenesRNA VirusesRecombination, GeneticSexTransduction, GeneticJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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M Stodolsky. 1974. Recipient gene duplication during generalized transduction.. https://doi.org/10.1093/genetics%2F78.3.809

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