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PubMed · 46254

Two distinct chemotactic factor inactivators in human serum.

G Till·P A Ward
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Abstract

The chemotactic factor inactivator (CFI) has been isolated from whole human serum by a combination of techniques including salt precipitation, anionic exchange, and gel filtration chromatography. Two inactivators have been obtained, a beta-globulin with a sedimentation velocity of approximately 7S and an alpha-globulin with a sedimentation velocity of approximately 4S. The former has a specificity for inactivation of the chemotactic activity associated with the C3 fragments, whereas the C5 chemotactic fragment is specifically inactivated by the alpha-globulin CFI. CFI in crude fractions of human serum is heat labile, time and temperature dependent for its activity, and pH dependent, expressing optimal activity at a pH range of 7.2 to 7.4.

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Alpha-GlobulinsAmmonium SulfateBeta-GlobulinsChemical PrecipitationChemotaxisChromatography, GelChromatography, Ion ExchangeComplement System ProteinsHumansHydrogen-Ion ConcentrationJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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G Till, P A Ward. 1975. Two distinct chemotactic factor inactivators in human serum.. https://pubmed.ncbi.nlm.nih.gov/46254/

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