RNA-dependent DNA polymerase activity of RNA tumor viruses. V. Rous sarcoma virus single-stranded RNA-DNA covalent hybrids in infected chicken embryo fibroblast cells.

J Leis; A Schincariol; R Ishizaki; J Hurwitz

doi:10.1128/jvi.15.3.484-489.1975

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RNA-dependent DNA polymerase activity of RNA tumor viruses. V. Rous sarcoma virus single-stranded RNA-DNA covalent hybrids in infected chicken embryo fibroblast cells.

J Leis·A Schincariol·R Ishizaki·J Hurwitz

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Abstract

RNA-DNA covalent hybrids containing viral RNA have been isolated from nuclear fractions of Rous sarcoma virus-infected chicken embryo fibroblast cells shortly after virus infection. The formation of covalent hybrid structures depends upon a functional reverse transcriptase in vivo, since its appearance in cells is temperature dependent when infected with Rous sarcoma virus mutant LA335, which contains a temperature-sensitive reverse transcriptase.

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Animals Avian Sarcoma Viruses Centrifugation, Isopycnic Centrifugation, Zonal Chick Embryo DNA Fibroblasts Mutation Nucleic Acid Hybridization RNA, Viral RNA-Directed DNA Polymerase Temperature Tritium Virus Replication Journal Article Research Support, U.S. Gov't, P.H.S.

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J Leis, A Schincariol, R Ishizaki, J Hurwitz. 1975. RNA-dependent DNA polymerase activity of RNA tumor viruses. V. Rous sarcoma virus single-stranded RNA-DNA covalent hybrids in infected chicken embryo fibroblast cells.. https://doi.org/10.1128/jvi.15.3.484-489.1975

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