PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 4629701

Polysaccharide produced by Anacystis nidulans: its ecological implication.

V K Sangar·P R Dugan
View original source ↗Export metadata ↓

Abstract

An extracellular polysaccharide from Anacystis nidulans was extracted from cell-free medium. Analysis showed that the polysaccharide consisted of glucose, galactose, and mannose in the ratio of 60:14:20. The production of polysaccharide depends on the age of culture, the growth temperature, and the form of nitrogen available.

Explore related subjects

Cell-Free SystemChromatography, GasChromatography, PaperColorimetryCulture MediaCyanobacteriaFresh WaterGalactoseGlucoseHydrolysisMannoseNitratesPhytoplanktonPolysaccharidesQuaternary Ammonium CompoundsTemperatureTime FactorsUreaJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

V K Sangar, P R Dugan. 1972. Polysaccharide produced by Anacystis nidulans: its ecological implication.. https://doi.org/10.1128/am.24.5.732-734.1972

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2006

Use of signal sequences as an in situ removable sequence element to stimulate protein synthesis in cell-free extracts.

This study developed a method to boost the expression of recombinant proteins in a cell-free protein synthesis system without leaving additional amino acid residues. It was found that the nucleotide sequences of the signal peptides serve as an efficient downstream box to stimulate protein synthesis when they were fused upstream of the target genes. The extent of stimulation was critically affected by the identity of the second codons of the signal sequences. Moreover, the yield of the synthesized protein was enhanced by as much as 10 times in the presence of an optimal second codon. The signal peptides were in situ cleaved and the target proteins were produced in their native sizes by carrying out the cell-free synthesis reactions in the presence of Triton X-100, most likely through the activation of signal peptidase in the S30 extract. The amplification of the template DNA and the addition of the signal sequences were accomplished by PCR. Hence, elevated levels of recombinant proteins were generated within several hours.

Cell-Free System↗
PubMed2006

Specific interactions with intra- and intermolecular G-quadruplex DNA structures by hydrosoluble coronene derivatives: a new class of telomerase inhibitors.

In developing G-quadruplex interactive telomerase inhibitors two main features have to be taken into account: the hydrophobic interactions with the G-quartet plane and the electrostatic interactions with the negatively charged phosphates of the four grooves. In this paper, we report the synthesis of four hydrosoluble coronene derivatives, which are characterized by a large hydrophobic aromatic core and four orthogonal hydrophilic side chains. We have studied their ability to induce both inter- and intramolecular G-quadruplex structures and found a significant selectivity of all the coronene derivatives for the intramolecular G-quadruplex. The efficiency in inhibiting human telomerase has been evaluated in a cell-free system and the experimental results correlate with the relative affinities of these compounds for the G-quadruplex monomeric structure, as derived by molecular modelling simulations. Thus, the coronene derivatives can be considered as a new class of telomerase inhibitors, although further investigations are surely necessary to fully exploit their features.

Cell-Free System↗