PubMed · 4654261
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R L Kaufmann, F Hillenkamp, E Remy. 1972. [The laser microprobe].. https://pubmed.ncbi.nlm.nih.gov/4654261/
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This paper shows that under some conditions the control exerted by a part of a metabolic network (a pathway) on a flux or concentration in any other part can be described through a single (overall) control coefficient. This has the following implications: (i) the relative contributions of a pathway enzyme to the regulation of the pathway (output) flux and of any flux or concentration outside are identical; therefore, the control analysis of the pathway 'in isolation' allows one to determine the control exerted by any pathway enzyme on the rest of the cell by estimation of the control efficient of just one, arbitrarily chosen enzyme; (ii) the relative control of any two metabolic variables outside the pathway (measured as the ratio of the control coefficients over these two variables outside) is the same for all pathway enzymes. These properties allow one to substitute effectively a pathway by a single (super)reaction and make it possible to consider such a pathway as a metabolic unit within the cellular enzyme network.
Nuclear magnetic resonance (NMR) continues to be a useful tool for the study of cellular metabolism. A variety of NMR techniques have been developed or newly applied to the analysis of cell systems. Many of these techniques are particularly useful for the analysis of immobilized cell bioreactors. The use of several NMR techniques has been an integral part of recent comprehensive metabolic studies. Novel computer-based models and methods have been developed which may make NMR study of metabolism more accessible and powerful.
Our understanding of cell physiology has been helped greatly by viewing metabolism as a set of reactions catalysed by independent catalysts (enzymes) in ideal solutions. Yet the differences between this idealized cell and reality have strong implications for biochemical regulation and control. We show here that in the real cell an enzyme controls cell physiology in more than a single way. These different controlling modes in the real 'macroworld' can be related to one another by implementing a new type of control analysis, which is formulated in terms of the 'microworld' of the elemental processes.