PubMed · 46544
The source did not provide an abstract. Follow the original record for more information.
Explore connections, maps & timelines
P J Pead, J V Gostling. 1975-02-15. Letter: Immunoglobulin separation using Sephadex G-200.. https://doi.org/10.1016/s0140-6736(75)91315-x
Cite the original work for its findings. Save a collection to share your selection of sources.
KEEP EXPLORING
The fractional composition of immunoglobulin preparations produced by different manufacturing enterprises of this country has been studied by gel chromatography in columns packed with different carriers (Sephadex G-200 and ultragel AcA-34) and by high-performance liquid chromatography (HPLC). This study has revealed the nonstandard character of immunoglobulin preparations produced according to the same technological procedure (modified Cohn's method). The fractionation of immunoglobulins on different carriers with the use of different methods has yielded similar results confirmed by the statistical processing of the data. The results obtained in the study of the fractional composition of immunoglobulin preparations evidence that gel filtration with the use of ultragel and HPLC have greater resolving capacity in comparison with the method of gel filtration on traditionally used Sephadex G-200.
Explore the source record for details and available documents.
We report a case of a woman (58 years old) with persistent and elevated CK activities and without corresponding clinical signs. For seven months CK-B activity was determined above normal by an inhibition test. By exclusion chromatography, ion-exchange chromatography, electrophoresis of CK isoenzymes and immunological methods we were able to explain this phenomenon as Macro CK consisting of immunoglobulin G bound CK-BB.