PubMed · 4683982
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J S King. 1973. Radioimmunoassay.. https://pubmed.ncbi.nlm.nih.gov/4683982/
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This paper discusses the application of the data handling-graphics-statistics program Stata (Computing Resources Center, Santa Monica, CA) to radioimmunoassay. We have found that this program is more powerful and easier to use than a spreadsheet for analyzing various kinds of laboratory data generated from chromatography, radiolabeling experiments, enzyme-linked immunosorbent assays, and radioimmunoassays, to name several examples. Data from a radioimmunoassay procedure, originally analyzed using a spreadsheet, Lotus 1-2-3, have been processed with Stata. Simple programs (batch files) have been devised for computations and graphics. The original data and a comparison of results are presented.
A simple radioimmunoassay (RIA) technique for measurement of specific activity of radiolabelled antigen is described. The technique involves setting up of a conventional RIA using multiple amounts of radiolabelled antigen as the unknown substance together with several RIA tubes containing the same amount of radiolabelled antigen with various known amounts of stable carrier. The known and unknown substances are equated at identical B/F ratio which would give the amounts of radiolabelled antigen. Counting the radioactivity in a counter with known efficiency gives its radioactive strength.