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A capillary diffusion method for determining hemolytic complement activity in undiluted serum .

J A Brockman·N Bauman
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Abstract

A method is presented for measuring the hemolytic activity of undiluted guinea pig serum. Undiluted serum is placed in contact with a column of sensitized sheep red blood cells in an agar matrix in a capillary tube. After incubation, the length of the zone of lysis is measured. Comparison with a standard curve prepared from dilutions of a pool of normal serum allows relative quantitation. Because the serum is not diluted, this method allows detection of reversible inhibitors of the complement system.

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AnimalsComplement ActivationComplement Inactivator ProteinsDiffusionGuinea PigsHemolysisJournal Article

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J A Brockman, N Bauman. 1979. A capillary diffusion method for determining hemolytic complement activity in undiluted serum .. https://doi.org/10.1016/0022-1759(79)90212-6

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