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PubMed · 4735791

Temperature variation of polyribonucleotide conformation by an interaction with basic globular proteins.

D A Stevenson·J W Preiss
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Abstract

Chicken lysozyme interacts with polyribonucleotides to form large light-scattering centers. The size of these is critically dependent on conformation of the polynucleotides. Hence, the interaction provides a very sensitive method of determining temperature intervals within which the polynucleotides change from multistranded, stacked-base structures to single-stranded, random coils. This change can be observed with the unaided eye, especially when concentrations of polynucleotides are of the order of a few micrograms per milliliter.

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Adenine NucleotidesAnimalsChickensCytosine NucleotidesGuanine NucleotidesInosine NucleotidesMuramidaseNucleic Acid ConformationNucleic Acid DenaturationPancreasPoly UPolynucleotidesRNA, TransferRibonucleasesRibonucleotidesScattering, RadiationTemperatureYeastsJournal Article

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BibTeXRIS

D A Stevenson, J W Preiss. 1973. Temperature variation of polyribonucleotide conformation by an interaction with basic globular proteins.. https://doi.org/10.1016/s0006-3495(73)86000-x

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