Protected nucleotide sequences in nuclear ribonucleoprotein.

L H Augenlicht

doi:10.1021/bi00584a022

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PubMed · 476087

Protected nucleotide sequences in nuclear ribonucleoprotein.

L H Augenlicht

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Abstract

The rapidly labeled nuclear ribonucleic acid in human carcinoma cells which is protected by protein from digestion by staphylococcal nuclease (EC 3.1.4.7) has been investigated. A simple and discrete sequence specificity was not found, but the protected RNA fragments are rich in G + C and were shown by fingerprinting to comprise a nonrandom subset of all heterogeneous nuclear ribonucleic acid (hnRNA) sequences enriched in the sequences AGC, GGC, AGGC, and GAGC. There was no detectable enrichment for dougble-stranded RNA in the protected fraction. These data provide the first evidence that the association of any protein with hnRNA is nonrandom with respect to nucleotide sequence.

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Base Sequence Cell Line Cell Nucleus Chromatin Colonic Neoplasms Humans Micrococcal Nuclease Nucleoproteins RNA, Heterogeneous Nuclear Ribonucleoproteins Ribonucleotides Journal Article Research Support, U.S. Gov't, P.H.S.

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L H Augenlicht. 1979-08-21. Protected nucleotide sequences in nuclear ribonucleoprotein.. https://doi.org/10.1021/bi00584a022

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